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随着超短脉冲激光的谱及,使用多光子激励法的扫描型荧光显微镜已经引起关注。以前,这类装置主要形式是把超短脉冲激光器组合到反射镜式扫描显微镜上。本文介绍的多光子显微镜使用透镜阵列和CCD摄像机,实现了以高于视频扫描的频率对荧光图像进行观察。光源使用的是钛蓝宝石激光器,其输出光束经扩束后,通过直径460μm、焦距6μm。的透镜阵列导入到显微镜内,这种透镜阵列是在熔石英盘上用刻蚀法制造,作为物镜使用的,数值孔径1.2的水浸透镜和数值孔径1.4的油浸透镜。通过CCD来观察试样发出的荧光。微阵列透镜各个透镜以等间隔螺线状配置,总体上呈最
With the ultrashort pulse laser spectrum and the use of multi-photon excitation scanning fluorescence microscope has drawn attention. Previously, the primary form of such devices was the combination of an ultra-short pulse laser with a mirror-type scanning microscope. This article describes the use of multi-photon microscope lens array and CCD cameras, to achieve higher than the frequency of video scanning of fluorescence images were observed. The light source uses a titanium sapphire laser, the output beam through the beam expander, through a diameter of 460μm, focal length 6μm. The lens array was introduced into a microscope which was fabricated on a fused silica disk by etching and used as an objective lens, a water immersion lens with a numerical aperture of 1.2 and an oil immersion lens with a numerical aperture of 1.4. Through the CCD to observe the fluorescence emitted by the sample. Each lens of the microarray lens is spirally arranged at equal intervals, and is generally the most