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目的了解陕西省克山病病区粮食和人群硒水平。方法按整群分层抽样的方法,于2006~2007年,在陕西省克山病区范围内抽取6个病区县(区)的12个自然村作为调查点,采集调查点居民户自产主食粮食小麦和玉米515份,克山病患者和病区健康人发样307份、全血248份及非病区健康人血样64份,用2,3-二氨基萘荧光法、原子荧光法和化学比色法分别测定粮食、发硒、全血硒含量和谷胱甘肽过氧化物酶(GSH-Px)活力。结果病区居民粮食(小麦、玉米)硒含量为(0.045±0.036)和(0.035±0.025)mg/kg,克山病患者发硒含量(0.376±0.091)mg/kg低于病区健康人(0.384±0.077)mg/kg,差异无统计学意义(P>0.05);克山病患者全血硒含量(0.071±0.017)mg/L低于病区健康人(0.077±0.017)mg/L,差异有统计学意义(P<0.05),病区健康人明显低于非病区健康人(0.090±0.016)mg/L,差异有统计学意义(P<0.01);克山病患者GSH-Px(75.16±12.10)U/L低于病区健康人(85.70±14.66)U/L,差异有统计学意义(P<0.05)。结论病区粮食和人群硒水平明显升高,与生活水平的提高和膳食结构的明显改善有关。
Objective To understand the selenium levels of food and population in Keshan disease area of Shaanxi Province. Methods According to cluster stratified sampling method, from 2006 to 2007, 12 natural villages in 6 ward counties (districts) were sampled from Keshan Ward in Shaanxi Province as survey sites. 515 samples of grain wheat and corn, 307 samples of Keshan disease and healthy ward samples, 248 whole blood samples and 64 blood samples of non-ward healthy people, using 2,3-diaminonaphthalene fluorescence method, atomic fluorescence method and The contents of selenium, total selenium and glutathione peroxidase (GSH-Px) in grain, hair selenium and whole blood were determined by chemical colorimetry. Results The selenium content of grain (wheat and corn) in ward residents was (0.045 ± 0.036) and (0.035 ± 0.025) mg / kg, and that in Keshan disease patients was 0.376 ± 0.091 mg / kg 0.384 ± 0.077) mg / kg, the difference was not statistically significant (P> 0.05). The Se content of whole blood in Keshan disease patients was lower than that in healthy people (0.077 ± 0.017) mg / L The difference was statistically significant (P <0.05), the healthy people in the ward were significantly lower than those in the non-ward healthy people (0.090 ± 0.016) mg / L, the difference was statistically significant (P0.01); GSH-Px (75.16 ± 12.10) U / L was lower than that of healthy people in Ward (85.70 ± 14.66) U / L, the difference was statistically significant (P <0.05). Conclusion The selenium levels of food and people in the ward are significantly increased, which is related to the improvement of living standards and the significant improvement of dietary structure.