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目的建立复方利胆解毒颗粒中绿原酸和连翘酯苷A的定量测定方法。方法采用HPLC法,Diamonsil-C18柱(250mm×4.6 mm,5μm),以甲醇-0.05%磷酸水(33∶67)为流动相,在330 nm检测波长下同时对复方利胆解毒颗粒中有效成分绿原酸和连翘酯苷A进行定量测定。结果绿原酸在0.044 72～0.894 4μg线性关系良好,r=1.000 0,平均回收率为98.92%,RSD为1.56%;连翘酯苷A在0.052 16～1.043 2μg线性关系良好,r=0.999 9;平均回收率为98.36%,RSD为1.44%。结论本方法专属性强、简便、准确,可有效控制该制剂的质量。
Objective To establish a method for the quantitative determination of chlorogenic acid and forsythoside A in Compound Lidan Jiedu Granules. Methods HPLC was performed on a Diamonsil-C18 column (250 mm × 4.6 mm, 5 μm) with methanol-0.05% phosphoric acid (33:67) as the mobile phase. The active components Chlorogenic acid and forsythoside A quantitative determination. Results The linear relationship of chlorogenic acid between 0.044 72 and 0.894 4μg was good, with the average recovery of 98.92% and the RSD of 1.56%. The calibration curve of forsythiaside A was good at 0.052 16 ~ 1.043 2μg, r = 0.999 9 ; The average recovery was 98.36%, RSD was 1.44%. Conclusion This method is specific, simple and accurate, which can effectively control the quality of the preparation.