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参照东营市孤岛地区土壤石油、盐渍化污染现状,通过人工模拟污染土样的方法分别研究了盐渍土壤中不同浓度石油对土壤微生物呼吸作用强度、过氧化氢酶活性、脲酶活性的影响。结果表明:培养第15、30、45天时,土壤微生物呼吸作用强度随着石油浓度的不断增大而增大,与土壤中石油的浓度呈现出明显的正相关,相关系数分别为0.889(P<0.05)、0.998(P<0.1)、0.964(P<0.1);通过对土壤酶活性的分析表明,随着石油浓度的不断增大,土壤过氧化氢酶活性呈现出先增大后减小的变化规律,当石油浓度在1 000、2 000 mg/kg之间时,此时过氧化氢酶活性最高,没有明显相关性;培养第1、15、30、45天时,土壤微生物脲酶活性随着石油浓度的不断增大而减小,与土壤中石油的浓度呈现出明显的负相关,相关系数分别为-0.933(P<0.05)、-0.890(P<0.05)、-0.898(P<0.05)、-0.951(P<0.05)。土壤呼吸作用强度和土壤脲酶活性可以作为表征东营孤岛盐渍土壤石油污染程度的敏感生物学和生化指标。
According to the status quo of soil petroleum and salinization in Gudao, Dongying, the effects of different concentrations of petroleum on the respiration intensity, catalase activity and urease activity of soil microorganisms were studied by simulated soil samples. The results showed that the respiration intensity of soil microorganisms increased with the increase of oil concentration on the 15th, 30th and 45th days of culture, and showed a significant positive correlation with the petroleum concentration in soil, the correlation coefficients were 0.889 (P <0.05 ), 0.998 (P <0.1), 0.964 (P <0.1). The analysis of soil enzyme activity showed that with the increase of petroleum concentration, the activity of soil catalase firstly increased and then decreased , When the oil concentration is between 1 000 and 2 000 mg / kg, the catalase activity is the highest at this time, and there is no obvious correlation between them. On the 1st, 15th, 30th and 45th days of culture, the soil urease activity changes with the oil concentration (P <0.05), -0.890 (P <0.05), -0.898 (P <0.05), -0.951 (P <0.05), and the correlation coefficient was -0.933 (P <0.05). Soil respiration intensity and soil urease activity can be used as a sensitive biological and biochemical index to characterize the degree of oil pollution in saline soil of Dongying Island.