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本文探讨了辣椒疫病生防菌普城沙雷菌A21-4在辣椒根际的定殖能力及其对辣椒的促生长和诱导抗病性影响。以108cfu·mL-1A21-4菌液处理辣椒苗,A21-4能够有效地定殖在辣椒根际土壤和辣椒根部,移栽第30 d在根际土壤和辣椒根部均保持106cfu·g-1以上定殖密度。辣椒根际土壤中A21-4的定殖密度和病原菌存在与否无显著差异,而辣椒根部A21-4的定殖密度在病原菌存在时显著高于没有病原菌的;A21-4处理有效促进了辣椒地上部和根部的各项生育指标,同时,显著提高了辣椒叶绿素含量和根系活力。A21-4处理的辣椒苗叶绿素含量和根系活力比对照各提高86.1%和481.8%;经A21-4处理后,辣椒根部和叶部的SOD、POD和PAL活性明显提高,辣椒根部的SOD、POD和PAL活性峰值分别比对照提高44.7%、64.2%和77.0%,辣椒叶部SOD、POD和PAL活性峰值分别比对照提高27.9%、134.9%和87.0%;此外,A21-4浸根处理还能够提高辣椒叶部对辣椒疫霉菌的抗性。
In this paper, the ability of colonization of Capsicum annuum A21-4 in pepper capsicum and its effect on promoting growth and inducing disease resistance of pepper were explored. Pepper seedlings were treated with 108cfu · mL-1A21-4 bacterium solution. A21-4 could colonize the rhizosphere soil and pepper root effectively, and rhizosphere soil and pepper roots at the 30th day after transplanting maintained 106cfu · g-1 Settling density above. The colonization density of A21-4 in rhizosphere soil of pepper and the existence of pathogen were not significantly different. However, the colonization density of A21-4 in pepper was significantly higher than that without pathogen in the presence of pathogen; A21-4 treatment effectively promoted the growth of pepper Aboveground and root of the reproductive indicators, at the same time, significantly increased pepper chlorophyll content and root activity. A21-4 treatment increased the chlorophyll content and root vigor of pepper seedlings by 86.1% and 481.8% respectively compared with the control; After A21-4 treatment, the activities of SOD, POD and PAL in roots and leaves of pepper increased significantly, while the activities of SOD, POD And PAL peak increased by 44.7%, 64.2% and 77.0%, respectively. The peak values of SOD, POD and PAL activity in pepper leaves were increased by 27.9%, 134.9% and 87.0%, respectively. In addition, Improve pepper leaf Phytophthora capsici resistance.