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从我国不同省份红麻病区采样分离获得16个炭疽病菌株,选有代表性的5个菌株,经产孢量和致病力测定显示,m-XS1菌株产孢量最多,对红麻侵染力最强,以伤口侵入为主,并能侵染老叶。进一步用m-XS1菌株,在温室里采用针伤茎叶+涂抹孢子和健株喷雾孢子悬液,对不同品种进行接种试验,结果看出,室内高湿下参试品种喷雾接种能充分发病,3个品种表现近似免疫,所得病情指数与伤茎叶接种之间相关系数r=0.774,呈极显著正相关。在大田采用以上2种方法接种160份红麻种质,结果表明伤茎叶接种中7份种质表现高抗,可为红麻杂交亲本选配提供可靠抗源。
Sixteen anthracnose isolates were obtained from samples of kenaf in different provinces in our country, and five representative strains were selected. The results of sporulation and pathogenicity showed that m-XS1 had the most sporulation, Strongest strongest invaders to the wounds, and can infect the old leaves. The m-XS1 strains were further used to inoculate spores and spores of healthy volunteers in the greenhouses and the inoculation experiments of different varieties were carried out. The results showed that spray inoculation could be fully induced in indoor high humidity, The three breeds showed similar immunity, and the correlation coefficient r = 0.774 between the obtained disease index and injured stem leaf inoculation was extremely significant positive correlation. In the field, 160 kenaf germplasms were inoculated by the above two methods, and the results showed that 7 germplasms in the injured stem leaf inoculation showed high resistance, which could provide a reliable source of resistance for kenaf hybrid parents.