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采用温室模拟Na Cl盐胁迫试验方法,设置5种Na Cl浓度即0%(CK)、0.2%、0.4%、0.6%、0.8%,对5份无芒雀麦种质材料叶绿素含量(Chl)、相对电导率(REC)、超氧化物歧化酶活性(SOD)、丙二醛含量(Pro)等11项指标进行测定,对各单项指标的耐盐系数进行主成分分析、隶属函数法和逐步回归等方法进行无芒雀麦苗期耐盐性综合评价。结果表明:根据耐盐综合评价值D值得出材料耐盐性排序:ZXY05P-171>ZXY05P-604>ZXY05P-629>ZXY06P-2592>ZXY05P-735,筛选出叶绿素含量(Chl)、脯氨酸含量(Pro)、过氧化物酶活性(POD)、丙二醛含量(MDA)4个无芒雀麦苗期耐盐性评价指标;5份材料耐盐性预测回归模型:D=-4.344+6.158Chl+0.076Pro-1.591POD-0.129MDA,为无芒雀麦苗期耐盐种质材料评价及新品种选育提供科学理论依据。
The chlorophyll content (Chl) of 5 species of Bromus inermis germplasm were determined by using NaCl salt stress test in greenhouse. Five kinds of NaCl concentrations were set as 0% (CK), 0.2%, 0.4%, 0.6% and 0.8% , Relative conductivity (REC), superoxide dismutase activity (SOD) and malondialdehyde content (Pro) were determined. Principal component analysis, membership function and stepwise regression were used to analyze the salt tolerance of each index Regression and other methods for the comprehensive evaluation of salt-tolerance of Brome grass. The results show that the order of salt tolerance of materials is ZXY05P-171> ZXY05P-604> ZXY05P-629> ZXY06P-2592> ZXY05P-735 according to the value of salt tolerance comprehensive evaluation value D. The chlorophyll content (Chl), proline content (Pro), peroxidase activity (POD) and malondialdehyde (MDA) in the seedlings of four Bromegrass seedlings. The regression model of five salt-tolerant materials was D = -4.344 + 6.158 Chl + 0.076Pro-1.591POD-0.129MDA, which provided a scientific basis for evaluation of salt-tolerant germplasm resources and selection of new varieties in seedling stage.