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通过γ射线辐射诱变籼稻9311和中恢8015分别获得了2个类病变突变体g303和g342。g303播种4~5周叶片开始出现黄褐色斑点。随着植株的生长发育,至成熟期病斑几乎布满整个植株。与野生型相比,突变体g303株高、结实率、千粒重显著下降,叶绿素含量、光合速率显著降低。遗传分析表明,g303类病变性状由一对隐性基因控制。利用g303/日本晴F2群体进行定位,将突变基因定位于第12染色体标记InD10和InD12之间,遗传距离分别为0.19cM和0.76cM。测序分析发现,该基因与SL基因等位,其中g303和g342分别在编码区第572位和第1206位发生单碱基缺失,导致翻译提前终止。实时RT-PCR结果显示抗病相关基因在突变体g303中表达量显著上升,说明该基因突变很可能激活了防卫反应。
Two mutant strains g303 and g342 were obtained by γ-ray irradiation in indica rice 9311 and Zhonghui 8015, respectively. g303 sowing 4 to 5 weeks leaves began to appear brown spots. With the growth and development of plants, the lesion almost covered the whole plant up to maturity. Compared with the wild type, the plant height, seed setting rate and 1000-grain weight of mutant g303 decreased significantly, and the content of chlorophyll and photosynthetic rate decreased significantly. Genetic analysis showed that the g303-like lesion was controlled by a pair of recessive genes. The F2 population of g303 / Nipponbare was used for mapping. The mutant gene was located between chromosome 12 and InD12 on chromosome 12 with genetic distances of 0.19cM and 0.76cM, respectively. Sequencing analysis revealed that the gene was allelic to the SL gene, of which g303 and g342 were single-base deletion at positions 572 and 1206 of the coding region respectively, leading to early termination of translation. Real-time RT-PCR results showed that the expression of disease-related genes in g303 mutant significantly increased, indicating that the gene mutation is likely to activate the defensive response.