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[目的]研究氧化苦参碱对体外乙醛造模后的肝星状细胞(HSC)增殖抑制及促凋亡作用的影响,探讨该药抗酒精性肝纤维化的作用机制。[方法]选取乙醛造模后HSC-T6为体外模型,以四甲基固氮唑盐(MTT)法分别检测800、400、200、100μg/ml浓度氧化苦参碱药物血清对HSC-T6作用48 h后的抑制情况,找出最佳含药浓度;根据以上结果,以流式细胞仪检测HSC-T6的促凋亡作用。[结果]800μg/ml氧化苦参碱对HSC-T6细胞生长的抑制率最高,达到51.31%,与模型组比较差异有统计学意义(P<0.01)。碘化丙啶染色流式细胞分析:800μg/ml药物血清作用HSC-T6,其在G0/G1、apop期细胞比例较模型组明显上升,S期细胞明显下降。[结论]氧化苦参碱可明显抑制HSC-T6增殖和诱导其调亡。
[Objective] To investigate the effect of oxymatrine on proliferation and apoptosis of hepatic stellate cells (HSC) induced by acetaldehyde in vitro and to explore the mechanism of action of oxymatrine on alcoholic liver fibrosis. [Methods] HSC-T6 was selected as the model of HSC-T6 after the formation of acetaldehyde. The effects of oxymatrine serum at concentration of 800, 400, 200, 100μg / ml on HSC-T6 were detected by MTT assay 48 h after the suppression of the situation to find the best drug concentration; based on the above results, the flow cytometry was used to detect the pro-apoptotic effect of HSC-T6. [Results] The inhibitory rate of 800μg / ml oxymatrine on HSC-T6 cells was the highest (51.31%), which was significantly different from that of model group (P <0.01). Propidium iodide staining flow cytometry analysis: 800μg / ml serum HSC-T6, the proportion of cells in G0 / G1, apop phase increased significantly compared with the model group, S phase cells decreased significantly. [Conclusion] Oxymatrine can obviously inhibit the proliferation and induce the apoptosis of HSC-T6.