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To observe the regulation of platelet-derived growth factor (PDGF) receptor-p in myocyte stimulated by angiotensin Ⅱ (AngII) at both integrated and cellular levels and reveal the signal transduction mechanism in cell, two kidneys, one clip (2K1C) renal hypertension were performed by placing a sliver clip around the left renal artery. Blood pressure and the ratio of left ventricular weight to body weight were measured at 4 and 8 weeks after operation. The content of Angll in heart was detected by radioimmunology assay; the protein level of PDGF receptor-β in heart was measured by Western blot analysis. The alteration of PDGF receptor-β stimulated by Angll and several inhibitors was observed on cultured neonatal rat ventricular myocyte (NRVM). The content of Angll in heart of 2K1C renal hypertensive rat at 4 and 8 weeks after operation was increased. Compared with sham group, 4 and 8 weeks after operation, PDGF receptor-p in heart of 2K1C group was upregulated by 100.3% and 127.1% (P < 0.05), respective
To observe the regulation of platelet-derived growth factor (PDGF) receptor-p in myocyte stimulated by angiotensin II (AngII) at both integrated and cellular levels and reveal the signal transduction mechanism in cell, two kidneys, one clip (2K1C) renal hypertension were performed by placing a sliver clip around the left renal artery. Blood content and the ratio of left ventricular weight to body weight were measured at 4 and 8 weeks after operation. The content of Angll in heart was detected by radioimmunology assay; the protein level of PDGF receptor-β in heart was measured by Western blot analysis. The alteration of PDGF receptor-β stimulated by Angll and several inhibitors was observed on cultured neonatal rat ventricular myocyte (NRVM). The content of Angll in heart of 2K1C renal hypertensive rat Compared with sham group, 4 and 8 weeks after operation, PDGF receptor-p in heart of 2K1C group was upregulated by 100.3% and 127.1% (P <0.05), respective