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针对当前免疫凝集微流控检测系统往往具有混合不可控、混合室结构复杂以及芯片复用率低等缺点,提出一种面向免疫凝集微流控检测的电控混沌混合方法。该方法在微流控芯片混合室壁面布置电极并施加混沌电场来驱动流体混合,通过分析选用Lorenz混沌系统作为混合控制电场,并制作了相应的混沌电场控制器。以类风湿因子在微流控检测系统中的免疫凝集检测为例,依据免疫凝集过程中2种样品液体不同的光学特性,采用吸光度检测法对此混沌混合方法实现的混合效果进行了检测验证。结果表明,该方法使得系统微混合室与受控流体系统体现出较高的控制性能,其相关系数为0.73,且在保证免疫凝集检测精度的条件下检测时间缩短48%,而芯片复用率也提高约7倍。
In view of the disadvantages of current immune coagulation microfluidic detection systems, such as uncontrollable mixing, complicated mixing chamber structure and low chip reusability, an electrically controlled chaotic hybrid method for immune coagulation-based microfluidic detection is proposed. In the method, electrodes are arranged on the wall of the microfluidic chip mixing chamber and a chaotic electric field is applied to drive fluid mixing. The Lorenz chaotic system is chosen as hybrid control electric field by analysis and a corresponding chaotic electric field controller is fabricated. Taking the immune agglutination test of rheumatoid factor in the microfluidic detection system as an example, the hybrid effect achieved by the chaotic hybridization method was tested by absorbance detection method according to the different optical properties of the two sample liquids in the process of immune agglutination. The results show that this method can make the system micro-mixing chamber and the controlled fluid system show higher control performance with a correlation coefficient of 0.73, and the detection time is shortened by 48% under the condition of ensuring the accuracy of the immune agglutination detection. The chip multiplexing rate Also increased about 7 times.