论文部分内容阅读
目的:探讨麻芥平喘巴布剂在细胞水平对Ⅰ型变态反应所引起的支气管哮喘的疗效机制。方法:用MTT比色法测定不同浓度麻芥平喘提取物对RBL-2H3细胞活化增殖的影响。根据MTT比色法结果选择麻芥平喘提取物5个不同的实验浓度,阳性对照组选用色甘酸钠。以C48/80刺激RBL-2H3细胞活化脱颗粒。通过ELISA法检测不同浓度药物对致敏的RBL-2H3细胞活化脱颗粒释放TNF-α及组胺的影响。结果:高浓度麻芥平喘提取物对RBL-2H3细胞增殖有抑制作用,IC50值为7.5 mg/m L。较高浓度麻芥平喘提取物可抑制激活的RBL-2H3细胞释放TNF-α和组胺,且其抑制作用强于色甘酸钠0.1 mg/m L组。RBL-2H3细胞激活后释放TNF-α抑制率与麻芥平喘提取物浓度的对数呈正相关(P=0.002<0.01)。结论:麻芥平喘巴布剂提取物可有效抑制经C48/80激活的RBL-2H3细胞脱颗粒释放TNF-α及组胺,这可能是麻芥平喘巴布剂抑制哮喘I型变态反应的机制之一。
OBJECTIVE: To investigate the therapeutic mechanism of hemp mustard and asthma pus agent on bronchial asthma caused by type Ⅰ allergy at the cellular level. Methods: MTT assay was used to determine the effect of different concentrations of Radix Astragali extract on the activation and proliferation of RBL-2H3 cells. According to MTT colorimetric results, five different experimental concentrations were selected for the astragalus extract, and the positive control group was cromolyn sodium. RBL-2H3 cells were stimulated with C48 / 80 to activate degranulation. The effects of different concentrations of drugs on the activation and degranulation of TNF-α and histamine in sensitized RBL-2H3 cells were detected by ELISA. Results: High concentration Herba asthma extract inhibited the proliferation of RBL-2H3 cells with IC50 of 7.5 mg / m L. The higher concentrations of Radix Astragali extract could inhibit the release of TNF-α and histamine from activated RBL-2H3 cells, and its inhibitory effect was stronger than that of cromolyn sodium 0.1 mg / m L group. The inhibition of TNF-α release from RBL-2H3 cells was positively correlated with the logarithm of the concentration of the extract of astragalus mongholicus (P = 0.002 <0.01). CONCLUSION: The extract of astragalus palmatum and pantoprazole can effectively inhibit the degranulation of TNF-α and histamine by C48 / 80-activated RBL-2H3 cells, which may be caused by the inhibition of asthma type I allergic reaction One of the mechanisms.