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[目的]探讨三黄汤灌肠对三硝基苯磺酸(TNBS)诱导溃疡性结肠炎(UC)模型大鼠的疗效。[方法]采用TNBS诱导制备UC大鼠模型45只,随机分为模型组、阳性对照组及中药治疗组,各15只,并选未造模大鼠15只作为正常对照组。各组均灌肠给药,模型组和正常对照组予0.9%氯化钠注射液,阳性对照组予柳氮磺吡啶0.5 g/kg,中药治疗组予三黄汤浓煎剂60 g/kg,各组均按10 ml/kg每日灌肠1次。给药时间从造模后第3天开始,连续7 d。第11天观察各组大鼠结肠黏膜损伤指数(CMDI)及黏膜病理组织学情况。[结果]中药治疗组结肠黏膜病理损伤明显改善,CMDI为(1.5±0.535)分,与模型组的(3.55±1.214)分比较差异有统计学意义(P<0.05);与阳性对照组的(2.63±1.188)分比较差异有统计学意义(P<0.05)。在肉眼及电镜下观察发现,中药治疗组大鼠结肠黏膜较模型组、阳性对照组明显改善。[结论]三黄汤灌肠对TNBS诱导的大鼠UC疗效确切,能显著改善结肠黏膜的充血水肿、溃疡、糜烂等。
[Objective] To investigate the effect of Sanhuang Tang enema on TNBS-induced ulcerative colitis (UC) model rats. [Methods] Forty-five UC rats were induced by TNBS and randomly divided into model group, positive control group and traditional Chinese medicine treatment group, 15 rats each. 15 non-model rats were selected as normal control group. Each group was enema, 0.9% sodium chloride injection in the model group and normal control group, the positive control group to sulfasalazine 0.5 g / kg, Chinese medicine treatment group to Sanhuang decoction 60 g / kg, each Group according to 10 ml / kg daily enema 1 times. Dosing from the first 3 days after modeling for 7 days. On the eleventh day, the colonic mucosal injury index (CMDI) and mucosal histopathology were observed in each group. [Results] The pathological changes of colonic mucosa in Chinese medicine group were significantly improved, the CMDI was (1.5 ± 0.535) points, which was significantly different from that in model group (3.55 ± 1.214) (P <0.05) 2.63 ± 1.188), the difference was statistically significant (P <0.05). The naked eye and electron microscopy showed that the Chinese medicine treatment group rats colonic mucosa than the model group, positive control group was significantly improved. [Conclusion] Sanhuang Decoction enema has definite curative effect on UC induced by TNBS in rats, and can significantly improve congestion, edema, ulceration and erosion of colonic mucosa.