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目的:建立高效液相色谱法测定人血浆中贝凡洛尔的浓度。方法:空白血浆加贝凡洛尔和内标普萘洛尔,液液萃取法进行提取,然后用HPLC进行分析。采用Plastisil ODS柱(250 mm×4.6 mm,5μm),流动相为乙腈-10 mmol甲酸胺水溶液(含0.2%甲酸)(35∶65),流速为1 mL.min-1,检测波长为280nm,柱温40℃,进样量为20μL。结果:本方法线性范围是15.5~1 984 ng.mL-1,r2=0.999 9,最小检出浓度(LLOQ)为15.5 ng.mL-1,绝对回收率均在86%以上,相对回收率在94%~98%之间,日内、日间RSD均小于15%。结论:本方法经济、简便、灵敏,适用于贝凡洛尔血药浓度检测和药动学研究。
Objective: To establish a HPLC method for the determination of bevantolol in human plasma. Methods: Blank plasma plus bevantolol and internal standard propranolol were extracted by liquid-liquid extraction and then analyzed by HPLC. Plastisil ODS column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase was acetonitrile-10 mmol formic acid aqueous solution (containing 0.2% formic acid) (35:65) at a flow rate of 1 mL · min- Column temperature 40 ℃, injection volume of 20μL. Results: The linear range of this method was 15.5-1 984 ng.mL-1, r2 = 0.999 9, the minimum detectable concentration (LLOQ) was 15.5 ng.mL-1, and the absolute recoveries were above 86% 94% ~ 98%, day, day RSD were less than 15%. Conclusion: The method is economical, simple and sensitive, suitable for the determination of Bevantolol blood concentration and pharmacokinetics.