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目的探讨胃腺癌原发灶及淋巴结转移灶与ｐ５３基因突变的关系。方法用ＰＣＲ－ＳＳＣＰ方法检测４７例胃腺癌及相关淋巴结转移灶ｐ５３基因第７外显子的突变。结果４７例原发灶有８例（１７％）、淋巴结转移灶有１８例（３８％）检测到ｐ５３基因突变。原发灶与转移灶同时发生突变的有７例（１５％）。胃癌原发灶ｐ５３基因突变与患者年龄、性别、病灶大小、肿瘤部位、肉眼类型、浸润深度、组织学类型、临床分期无关（Ｐ＞０．０５），与远处转移有关（Ｐ＜０．０１）。淋巴结转移灶ｐ５３基因突变与患者年龄、性别、病灶大小、肿瘤部位、肉眼类型、浸润深度无关（Ｐ＞０．０５，与组织学类型、远处转移及分期有关（Ｐ＜０．０５，Ｐ＜０．０１）。淋巴结转移灶ｐ５３基因突变高于原发灶（Ｐ＜０．０５）。结论 ｐ５３基因在转移时发生新突变，转移淋巴结具有更强的异质性。［
Objective To investigate the relationship between p53 gene mutation and primary tumor and lymph node metastasis in gastric adenocarcinoma. Methods The mutation of exon 7 of p53 gene in 47 cases of gastric adenocarcinoma and its associated lymph node metastasis was detected by PCR-SSCP. Results The p53 gene mutation was detected in 47 cases of primary tumor in 8 cases (17%) and in 18 cases (38%) of lymph node metastasis. There were 7 cases (15%) of primary and metastatic mutations simultaneously. The mutation of p53 gene in primary gastric cancer was not associated with age, sex, size of lesion, tumor location, type of macroscopic, depth of invasion, histological type, clinical stage (P> 0.05), but was associated with distant metastasis (P <0. 01). The p53 gene mutation in lymph node metastasis was not associated with age, sex, size of lesion, tumor location, type of macroscopic and depth of invasion (P> 0.05), but was associated with histological type, distant metastasis and staging (P <0.05, P <0.01) .The mutation of p53 gene in lymph node metastasis was higher than that in primary tumor (P <0.05) .Conclusion The p53 gene has a new mutation in metastasis and the metastatic lymph node has more heterogeneity [