论文部分内容阅读
转录因子Rex是一种广泛存在于革兰氏阳性菌,能够与NADH或者NAD~+直接结合响应胞内NADH/NAD~+的氧化还原传感器,与靶基因的结合可调节细胞内的多种生理代谢。NAD(H)是调节细胞能量代谢的必需辅酶,显示微生物细胞内的氧化还原状态。研究发现Rex的调节活性与细胞内NADH/NAD~+比率相关。需氧和厌氧菌属中Rex单体和复合物晶体结构的解析揭示了Rex、NADH/NAD~+和靶基因间的作用关系及调控机制。通过比较分析了不同菌株中Rex单体和复合物的晶体蛋白结构,并揭示了NADH/NAD~+对Rex调控活性的影响,进一步解析了Rex与碳和能量代谢、厌氧代谢、发酵、生物膜等之间的联系,并展望了Rex的研究和应用方向。
Rex, a transcription factor widely present in Gram-positive bacteria that binds directly to NADH or NAD + in response to intracellular NADH / NAD ~ +, binds to target genes that regulate various physiological metabolism. NAD (H) is an essential coenzyme that regulates the energy metabolism of cells and shows the redox status within microbial cells. The study found that the regulatory activity of Rex and intracellular NADH / NAD ~ + ratio. The crystal structure analysis of Rex monomer and complex in aerobic and anaerobic bacteria revealed the interaction and regulation of Rex, NADH / NAD ~ + and target genes. The crystal structure of Rex monomer and complex in different strains was analyzed and compared. The effect of NADH / NAD ~ + on the Rex regulatory activity was revealed. Rex was further analyzed for the relationship between Rex and carbon and energy metabolism, anaerobic metabolism, fermentation, Membrane, etc., and prospects of Rex’s research and application direction.