论文部分内容阅读
目的:探讨不同阶段应用丹参注射液对急性胰腺炎(acute panereatitis,AP)大鼠自由基水平的影响,从而探讨丹参注射液防治急性胰腺炎的机理。方法:30只雄性Wistar大鼠,随机分为空白对照组、模型组和丹参组,采用L-精氨酸腹腔注射方法诱导大鼠急性胰腺炎模型,丹参组于不同时间注射丹参注射液(尾静脉注射)。完成后2h,腹腔麻醉,经腹主动脉采血,离心分离血清,动态测定大鼠血清淀粉酶(AMY)、丙二醛(MDA)、超氧化物歧化酶(SOD),评估不同阶段注射丹参注射液对急性胰腺炎的防治作用。结果 :丹参组实验室指标血清淀粉酶(AMY、MDA、SOD)较对照组有明显差异,AP组AMY和MDA水平升高,SOD水平降低,与对照组比较有统计学差异(P<0.05);丹参治疗组在各个时间点都可降低AMY和MDA的水平(P<0.05),升高SOD水平(P<0.05)。结论:丹参通过降低损害因子MDA、升高保护因子SOD对AP有治疗作用,丹参注射液的早期应用对急性胰腺炎的防治作用效果最佳。
Objective: To investigate the effects of Danshen injection at different stages on free radicals in rats with acute pancreatitis (AP), and to explore the mechanism of Danshen injection in preventing and treating acute pancreatitis. Methods: Thirty male Wistar rats were randomly divided into blank control group, model group and Salvia miltiorrhiza group. Rats model of acute pancreatitis was induced by intraperitoneal injection of L-arginine and Danshen injection was injected at different times. Intravenous injection). 2h after completion, intraperitoneal anesthesia, abdominal aorta blood collection, serum was separated by centrifugation, dynamic determination of serum amylase (MDA), malondialdehyde (MDA), superoxide dismutase (SOD), assessment of different stages of injection Danshen injection Liquid on the prevention and treatment of acute pancreatitis. Results: There were significant differences in serum amylase (AMY, MDA, SOD) between the Salvia miltiorrhiza group and the control group. The levels of AMY and MDA in the AP group were increased and the SOD level was decreased. There was a statistically significant difference compared with the control group (P<0.05). Danshen treatment group can reduce AMY and MDA levels (P<0.05) and increase SOD levels at various time points (P<0.05). Conclusion: Salvia miltiorrhiza can reduce the damage factor MDA and increase the protective factor SOD to AP. The early application of Danshen injection has the best effect on the prevention and treatment of acute pancreatitis.