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目的探讨HPV分型检测在宫颈病变诊断中的临床价值。方法对2013年9月至2014年9月收治的80例宫颈病变患者行液基薄层细胞检测(TCT)和HPV分型检测,并对其检测进行分析。结果 80例患者中,TCT结果示ASC53例、LSIL20例、HSIL7例;HPV分型检测示HPV阳性49例,阳性率61.3%。检出的基因型有15种,分别是高危型HPV16、18、31、33、39、45、52、58、35、56和低危型HPV6、11、42、44及亚型CP8304;其中高危型HPV感染28例(占57.1%)、低危型HPV感染10例(占20.4%)、混合型HPV感染11例(占22.4%)。TCT结果与HPV检测阳性结果呈正相关,即随着TCT显示宫颈病变严重程度的增高,HPV检测阳性率亦上升;HPV检测阴性31例中,仅5例病理确诊为CIN。结论 HPV分型检测在临床诊断宫颈病变中具有极高的灵敏度,其检测结果的阴性预测值极高,值得推广使用。
Objective To investigate the clinical value of HPV typing in the diagnosis of cervical lesions. Methods Eighty cases of cervical lesions from September 2013 to September 2014 were examined by liquid-based thin-layer cytometry (TCT) and HPV typing, and their detection was analyzed. Results Among the 80 patients, the results of TCT showed ASC53 cases, LSIL20 cases and HSIL7 cases. The HPV type tests showed that HPV was positive in 49 cases, the positive rate was 61.3%. Fifteen genotypes were detected, which were high-risk HPV16,18,31,33,39,45,52,58,35,56 and low-risk HPV6,11,42,44 and subtype CP8304 respectively. Among them, high-risk There were 28 cases (57.1%) with HPV infection, 10 cases (20.4%) with low-risk HPV infection and 11 (22.4%) with HPV infection. TCT results and HPV test positive results were positively correlated, that is, as TCT showed increased severity of cervical lesions, HPV test positive rate also increased; HPV negative in 31 cases, only 5 cases of pathological diagnosis of CIN. Conclusion HPV genotyping has high sensitivity in clinical diagnosis of cervical lesions. The negative predictive value of HPV test results is extremely high and should be widely used.