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本研究采用微团培养观察了Ｖ２Ｏ５对大鼠胚胎中脑细胞增殖和分化的影响，旨在为进一步揭示其发育毒作用机理提供依据。结果显示，Ｖ２Ｏ５对体外中脑细胞的增殖和分化均有抑制作用，并有明显的剂量－反应关系。Ｖ２Ｏ５抑制中脑增殖和分化的ＩＣ５０分别为９１和６８μｍｏｌ／ｌ。体内／体外结合试验显示，Ｖ２Ｏ５在２０ｍｇ／ｋｇ时对细胞分化有明显的抑制作用，而在５０ｍｇ／ｋｇ时才对细胞增殖出现抑制作用，按照Ｒｅｎａｕｌｔ的分类标准，可判定Ｖ２Ｏ５是胚胎中脑细胞的特异抑制物
In this study, we investigated the effect of V2O5 on the proliferation and differentiation of rat embryonic mesencephalic cells using micellar culture in order to provide a basis for further revealing the mechanism of developmental toxicity. The results showed that V2O5 inhibited the proliferation and differentiation of midbrain cells in vitro and had a dose-response relationship. The IC50 of V2O5 inhibiting midbrain proliferation and differentiation were 91 and 68μmol / l, respectively. In vivo / in vitro binding assays showed that V2O5 at 2 0mg / kg significantly inhibited cell differentiation, while in 5 0mg / kg when cell proliferation was inhibited, according to Renault’s classification criteria, can be judged V2O5 is Specific inhibition of midbrain cells in embryos