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目的:探讨放疗、化疗单独及联合应用对脊髓产生的影响。方法:实验共分为4组,即对照组、单纯放疗组、单药化疗组及放化疗联合组。顺铂的剂量各为5mg/kg,单次给予;放射剂量为5Gy/次,采用6MeV的电子线,隔日1次照射大鼠胸段脊髓,总量为40Gy。照射结束后20周,免疫组化检测放疗、化疗及联合治疗时脊髓组织中p53蛋白的表达。结果:空白组、单纯放疗组、顺铂化疗组、顺铂化疗及放疗联合组p53阳性表达率分别为0、36.6%、43.3%和70.0%,联合组与其他3组比较,P<0.05。结论:顺铂加放疗联合组上调脊髓组织p53表达水平,提示放化疗联用可能加重脊髓损伤,对今后设计合理治疗方案有指导意义。
Objective: To investigate the radiotherapy, chemotherapy alone and in combination on the spinal cord production. Methods: The experiment was divided into 4 groups: control group, radiotherapy alone group, single drug chemotherapy group and radiotherapy and chemotherapy combination group. Cisplatin dose of 5mg / kg, a single administration; radiation dose of 5Gy / times, the use of 6MeV electron beam, once every other day thoracic spinal cord irradiation, the total amount of 40Gy. Twenty weeks after irradiation, immunohistochemistry was used to detect the expression of p53 protein in spinal cord tissue after radiotherapy, chemotherapy and combination therapy. Results: The positive rates of p53 in blank group, radiotherapy alone group, cisplatin chemotherapy group, cisplatin chemotherapy group and radiotherapy combined group were 0, 36.6%, 43.3% and 70.0%, respectively. The combined group was significantly lower than the other 3 groups (P <0.05). Conclusion: The combination of cisplatin plus radiotherapy up-regulated the expression of p53 in spinal cord tissues, suggesting that combination of radiotherapy and chemotherapy may aggravate spinal cord injury, which may be instructive for the design of rational treatment in the future.