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目的 分析乙肝患者HBVDNA前C/C区和基本核心启动子区部分DNA片断突变。方法 PCR扩增HBVDNAnt1735～196 5片段,将产物进行HBVDNA测序。结果 在6 8例乙肝患者中,突变阳性率4 8. 5 % ,点突变16 8个,频率前10位的是nt176 4 ,176 2 ,1799,176 6 ,1896 ,175 4 ,1899,176 8,1814及1913,还检出鲜见报道的192 3,192 2 ,190 7等点突变。5 4例慢性肝炎和10例肝炎肝硬化患者HBVDNAnt1896 ,176 4 ,176 2点突变阳性数分别为9,19,19和3,19,19,两者差别有统计学意义(P <0 . 0 1)。结论 提示PreC/C与BCP区基因突变可能与肝实质纤维化相关联;基因测序对芯片探针设计有着十分重要的指导价值和临床意义;HBVDNA突变发生率较高,且位点众多,基因突变分析对肝炎诊疗与流行病学研究有着十分重要的意义。
OBJECTIVE: To analyze the mutations in DNA fragments of pre-HBVDNA C / C region and basic core promoter region in hepatitis B patients. Methods PCR amplification of HBVDNAnt1735 ~ 196 5 fragments, the product was HBVDNA sequencing. Results Among 68 hepatitis B patients, the positive rate of mutation was 48.5% and the number of point mutations was 16.8. The top ten frequencies of the mutation were nt176 4, 176 2, 1799, 176 6, 1896, 175 4, 1899 and 176 8 , 1814 and 1913, but also rarely detected point mutations of 192 3,192 2 190 190 were detected. The positive numbers of HBVDNA18, 176 4, 176 2 mutations in 5 4 patients with chronic hepatitis and 10 patients with hepatitis cirrhosis were 9, 19, 19 and 3, 19 and 19, respectively, with significant difference (P <0. 0 1). Conclusions: PreC / C and BCP mutations may be associated with hepatic parenchymal fibrosis. Gene sequencing has very important guiding value and clinical significance for the design of chip probes. HBVDNA mutation has a high incidence and many loci, Analysis of the diagnosis and treatment of hepatitis epidemiology has very important significance.