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目的:探讨结肠癌组织中β-catenin和Nanog表达及其对复发转移的预测作用。方法:采用免疫组化方法检测80例结肠癌组织β-catenin和nanog表达,RT-PCR检测20例冷冻标本肿瘤及癌旁组织中Nanog表达。结果:在80例患者中,35例出现复发转移,β-catenin和Nanog在转移组中表达率分别为65.7%(23/35)和60.0%(21/35),在非转移组中分别为31.1%(14/45)和26.7%(12/45),差异有统计学意义(χ2=9.483,P=0.002;χ2=9.026,P=0.003)。结肠癌原发灶分化程度不同,β-catenin和Nanog表达存在差异,但是同T和N分期没有明显联系。生存分析结果显示,不同表达状态其远处转移出现时间差异有统计学意义(P=0.000 2),两者均为阳性表达其转移率明显高于均为阴性表达。RT-PCR分析下结果显示,20例肿瘤组织中Nanog阳性表达率为35.0%(7/20),明显高于癌旁组织,P=0.018。结论:肿瘤组织中β-catenin和Nanog表达同结肠癌术后复发转移发生相关,两者联合检测有助于评估肿瘤转移的可能。
OBJECTIVE: To investigate the expression of β-catenin and Nanog in colon carcinoma and its role in the prediction of recurrence and metastasis. Methods: The expression of β-catenin and nanog in 80 cases of colon cancer was detected by immunohistochemistry. The expression of Nanog was detected by RT-PCR in 20 cases of frozen specimens and adjacent tissues. Results: Of the 80 patients, 35 cases showed recurrence and metastasis. The expression rates of β-catenin and Nanog were 65.7% (23/35) and 60.0% (21/35) in the metastasis group, respectively 31.1% (14/45) and 26.7% (12/45), respectively. The difference was statistically significant (χ2 = 9.483, P = 0.002; χ2 = 9.026, P = 0.003). The differentiation degree of primary tumor in colon cancer is different, the expression of β-catenin and Nanog are different, but there is no obvious correlation with T and N staging. Survival analysis showed that there was significant difference in the time of distant metastasis between different expression states (P = 0.000 2). The positive rates of metastasis were significantly higher than those of negative expression. The results of RT-PCR showed that the positive expression rate of Nanog in 20 cases was 35.0% (7/20), which was significantly higher than that in paracancerous tissues (P = 0.018). Conclusion: The expression of β-catenin and Nanog in tumor tissue is related to the recurrence and metastasis of colon cancer. The combined detection of β-catenin and Nanog can be helpful to evaluate the possibility of tumor metastasis.