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目的分析2010-2012年河南省信阳息县<6岁健康儿童肠道病毒71型(EV71)中和抗体水平,了解河南省近几年EV71流行趋势,为防控EV71引起的手足口病流行提供科学依据。方法挑选2010-2012年信阳息县<6岁健康儿童血清标本254份,进行EV71标准毒株(阜阳毒株)的微量中和试验,用SPSS 17.0统计学软件分析EV71中和抗体阳性率、构成比和几何平均滴度,样本率的比较用χ2检验。结果2010-2012年信阳息县<6岁健康儿童EV71抗体总阳性率为68.90%,3年分别为75.56%、61.22%和71.21%,与当年本地区手足口病发病率呈正相关。<1岁、1~岁组EV71抗体阳性率和几何平均滴度均低于3~岁、5~6岁组,差异有统计学意义(χ2=18.59,P<0.01;t=0.47,P<0.05)。1~岁组几何平均滴度最低,为1∶25.18。结论 <2岁婴幼儿更易感染EV71,健康儿童血清EV71抗体阳性率与当年手足口病的发病率呈正相关。
Objective To analyze the neutralizing antibody level of enterovirus 71 (EV71) in healthy children from 6 years old in Xinyang County of Henan Province from 2010 to 2012, and to find out the epidemic trend of EV71 in Henan Province in recent years, and to provide prevention and control of EV71-induced HFMD epidemic Scientific basis. Methods A total of 254 serum samples from healthy children aged 6 years and older in Xinyang County from 2010 to 2012 were collected for micro neutralization test of EV71 standard strain (strain Fuyang). The positive rates of neutralizing antibodies against EV71 were analyzed by SPSS 17.0 statistical software, Ratio and geometric mean titer, sample rate comparison with χ2 test. Results The positive rate of EV71 antibody in healthy children aged 6 to 6 years in Xinyang County from 2010 to 2012 was 68.90%, 75.56%, 61.22% and 71.21% respectively in 3 years, which was positively correlated with the prevalence of HFMD in this area. The positive rates and geometric mean titers of EV71 antibodies in patients <1 year old and 1 ~ year old were lower than those of 3 ~ 5 years old and 6 years old (χ2 = 18.59, P <0.01; t = 0.05). The geometric mean titer of 1 ~ group was the lowest, 1:25.18. Conclusion Infants younger than 2 years are more likely to be infected with EV71 and the positive rate of serum EV71 antibody in normal children is positively correlated with the incidence of hand-foot-mouth disease.