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目的通过大鼠肠粘连动物模型,检测基质金属蛋白酶-1(MMP-1)和基质金属蛋白酶抑制物-1(TIMP-1)在肠粘连组织及正常组织中的表达,研究MMP-1和TIMP-1在肠粘连发病中的作用。方法选取SD雄性大鼠70只随机分为3组:10只作为正常组;观察组30只刮伤其回肠浆膜,创面滴无水酒精,钳夹盲肠系膜动脉制成损伤性肠粘连模型;对照组30只大鼠与观察组一样麻醉、开腹,但不动肠组织,直接关腹。观察组及对照组分别于造模后2、7、14d3个时相点各取10只大鼠,处死并分别取小肠浆膜层浆膜粘连面组织,应用免疫组化和图像分析的方法测定MMP-1和TIMP-1在肠粘连组织及正常肠组织中的蛋白表达。结果 (1)造模术后2d,观察组中MMP-1和TIMP-1的含量均升高(P均<0.01),MMP-1升至峰值。(2)造模术后7d,观察组MMP-1含量降低,而TIMP-1的含量升高至峰值,与术后2d及对照组相比,差异均有统计学意义(P均<0.01)。(3)造模术后14d,观察组MMP-1、TIMP-1的含量均下降,与术后2、7d及对照组相比,差异均有统计学意义(P均<0.01)。(4)对照组各时相点两两比较,无明显差异(P均>0.05)。结论 MMP-1、TIMP-1的活性变化是肠粘连形成的重要调节因素之一。
Objective To investigate the expression of matrix metalloproteinase-1 (MMP-1) and matrix metalloproteinase-1 (TIMP-1) in intestinal adhesion and normal tissues by animal model of intestinal adhesion, -1 in the pathogenesis of intestinal adhesion. Methods Seventy SD male rats were randomly divided into 3 groups: 10 as normal group; 30 rabbits in the observation group were scored with ileal serous membrane, Thirty rats in the control group were anesthetized and open the same way as the observation group, but did not move the intestine and directly closed the belly. In the observation group and the control group, 10 rats were taken at 3, 7, and 14 days after the model was established respectively. The tissues of serosal adhesions of the serosal serosa of the small intestine were sacrificed and examined by immunohistochemistry and image analysis The protein expression of MMP-1 and TIMP-1 in intestinal adhesion tissue and normal intestinal tissue. Results (1) The levels of MMP-1 and TIMP-1 in the observation group were both increased (P <0.01) two days after the model was established, and the MMP-1 peaked. (2) The levels of MMP-1 in the observation group decreased and the content of TIMP-1 increased to the peak value at 7 days after modeling, compared with the control group at 2 days after operation (all P <0.01) . (3) The levels of MMP-1 and TIMP-1 in the observation group decreased on the 14th day after modeling, and there were significant differences between the observation group and the control group on the 2nd and 7th day (all P <0.01). (4) There was no significant difference between the control group and each time point (P> 0.05). Conclusion The changes of MMP-1 and TIMP-1 activity are one of the important regulators of intestinal adhesion formation.