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以野生型和过表达ZmSKIP基因烟草为试材,研究了低温胁迫下过表达ZmSKIP对烟草抗氧化能力的影响。测定了不同低温处理时间下过表达ZmSKIP转基因烟草T3代植株和野生型植株抗氧化酶如超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性和丙二醛(MDA)含量以及相对电导率,结果表明,低温下,相对于野生型植株,转基因烟草具有较高的抗氧化酶活性和较低的相对电导率和MDA含量,说明过表达ZmSKIP提高了转基因植株的耐低温胁迫能力。
The effects of ZmSKIP over-expressing ZmSKIP on antioxidant capacity of tobacco were studied using wild-type and overexpression ZmSKIP-transgenic tobacco as test material. The activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and phosphoenolpyruvate dehydrogenase (ciprofloxacin) in transgenic plants of T3 generations and wild type plants overexpressing ZmSKIP transgenic tobacco at different low temperature treatments The results showed that transgenic tobacco had higher antioxidant enzyme activity and lower relative conductivity and MDA content than wild type plants at low temperature, indicating that overexpression of ZmSKIP increased Cold stress tolerance of transgenic plants.