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1引言用光度法测定蛋白质以其重视性好、灵敏度高而在临床检验和生化实验中得到应用。实验表明，硝基磺酚S在酸性条件下可与蛋白质作用生成蓝色复合物。此方法用于实际人血清样品中蛋白质的测定，结果准确，回收率高，方法简便。2实验方法向钻niL比色管中依次加入2．SInL硝基磺
1 Introduction Spectrophotometric determination of protein with its importance, sensitivity and clinical testing and biochemical experiments have been applied. Experiments show that nitrosulphol S can form a blue complex with the protein under acidic conditions. The method is applied to the determination of protein in real human serum samples with accurate results, high recovery rate and simple and convenient method. 2 experimental methods to drill niL colorimetric tube followed by adding 2. SInL Nitroso