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目的 观察短时氧惊厥沙土鼠脑海马各区神经元c-fos蛋白的表达.探讨氧惊厥时神经元激活部位.方法 18只健康雄性沙土鼠经观察和检疫1周后进行实验.按体质量采用随机数字法分为3组(正常对照组、短时氧惊厥组、压力对照组),每组3只.沙土鼠暴露在0.5 MPa压力的高压氧下,引起短时氧惊厥(首次惊厥5 min后,减压出舱)后应用LSAB染色法观察海马各区神经元c-fos蛋白的表达情况.结果 沙土鼠在发生短时氧惊厥后,用免疫组化染色的脑切片上发现海马各区神经元c-fos蛋白阳性表达,依次为CA1、CA2、CA4、CA3区.结论 海马是氧惊厥时神经元激活的部位之一,海马CA1区作用最强.“,”Objective To observe changes in the expression of c-fos protein in different regains of gerbil hippocampus following transient hyperbaric oxygen-induced convulsion(HBOC)and to explore neuronal localization of the HBOC.Methods Eighteen healthy mail gerbils were used and experiments were carried out after 1 week of observation and quarantine.Gerbils were divided by random number table into normal control,transient convulsion and hyperbaric oxygen(HBO)control groups with 3 gerbils in each group.Transient oxygen convulsion was induced by 0.5 MPa HBO(gerbils were taken out 5 min later following first convulsion),then morphological changes of neurons in hippocampas were obsered by LSAB staining.Results The expression of c-fos protein could be found in different regions of gerbil hippocampus following transient HBOC.The expression of c-fos protein was the highest in CA1 regain,and the expression decreased in CA2,CA4,CA3 regains in order.Conclusions Hippocampus is one of the neuronal localization of HBOC,and it is most infected in CA regain by HBOC.