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采用溶胶-凝胶法通过正硅酸四乙酯(TEOS)碱催化水解,在Fe3O4纳米粒子表面包裹氧化硅。利用生物倒置显微镜、场发射透射电镜、X射线衍射仪、激光粒度仪、振动样品磁强计对氧化硅/Fe3O4复合粒子的外貌、粒径及粒径分布、饱和磁化强度、化学成分进行了表征。结果表明,所制得的复合粒子性能良好,粒径在15 nm左右,饱和磁化强度为10.9 emu/g。用该磁性纳米复合粒子提取质粒DNA和基因组DNA取得良好的效果,可用于食品中致病菌的分析判定和疾病的基因诊断分析。
Sol-gel method was used to catalyze the hydrolysis of tetraethyl orthosilicate (TEOS) to encapsulate the surface of Fe3O4 nanoparticles. The appearance, particle size and particle size distribution, saturation magnetization and chemical composition of silica / Fe3O4 composite particles were characterized by using biological inverted microscope, field emission transmission electron microscope, X-ray diffraction, laser particle size analyzer and vibrating sample magnetometer . The results show that the prepared composite particles have a good performance with a particle size of about 15 nm and a saturation magnetization of 10.9 emu / g. The use of the magnetic nanocomposite particle extraction of plasmid DNA and genomic DNA obtained good results, can be used for the determination of pathogens in food and genetic diagnosis of the disease.