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目的:观察益心康泰对老龄SD大鼠精子密度和活率,以及血清超氧化物歧化酶(SOD)和丙二醛(MDA)的影响,并探讨其可能的作用机制。方法:将老年(24月龄)SD雄性大鼠随机分为空白组、十一酸睾酮组、益心康泰高、中、低剂量组共5组,根据体重,分别给予10 ml/kg生理盐水、4 mg/kg十一酸睾酮悬浊液、0.6 g/kg、0.3 g/kg、0.15 g/kg益心康泰悬浊液灌胃,1次/d,30 d后,取附睾,剥离脂肪、包膜,剪碎放入37℃恒温水浴锅中预热的生理盐水试管内,利用CASA精子自动分析系统(伟力)检测精子各项常规参数。取血查血清SOD和MDA含量。并行睾丸组织HE染色,在光镜下观察睾丸组织形态学变化。结果:与空白对照组相比,高、中、低剂量益心康泰均能显著提高老年大鼠的精子密度[分别为(152.3±24.0)×106/ml、(334.5±97.7)×106/ml、(302.7±158.9)×106/ml、(221.4±75.6)×106/ml,P<0.05]和活率[分别为(26.5±2.0)%、(35.5±5.9)%、(33.1±5.4)%、(28.4±2.1)%,高、中剂量与空白组相比P<0.01],同时,高剂量组可以显著提高血清SOD含量[(22.0±5.3)U/ml,P<0.01],降低MDA含量[(13.6±4.6)nmol/ml,P<0.01];睾丸组织学检测显示,给药组相对于空白组细胞增殖更为活跃,可见较多分裂期细胞,管腔内成熟精子数目较多,间质稀少,血管丰富,间质细胞发育良好。结论:益心康泰可以通过影响SOD和MDA的含量而改善老年SD大鼠的生精功能。
Objective: To observe the effects of Yixinkangtai on the sperm density, viability, serum superoxide dismutase (SOD) and malondialdehyde (MDA) in aged SD rats and to explore its possible mechanism. Methods: Aged (24 months old) SD male rats were randomly divided into blank group, testosterone undecanoate group, Yixintangtai high, medium and low dose group of 5 groups, according to body weight, were given 10 ml / kg saline , 4 mg / kg testosterone undecanoate suspension, 0.6 g / kg, 0.3 g / kg, 0.15 g / kg Yixintangtai suspension orally, 1 times / d, 30 d, , Wrapped, cut into 37 ° C water bath preheated saline test tube, the use of CASA sperm automatic analysis system (Weili) to detect the sperm of the conventional parameters. Take blood to check serum SOD and MDA content. Parallel testicular tissue HE staining, observed under light microscope, testicular histological changes. Results: Compared with the blank control group, Yixintangtai high, medium and low doses significantly increased the sperm density of the aged rats [(152.3 ± 24.0) × 106 / ml and (334.5 ± 97.7) × 106 / ml , (302.7 ± 158.9) × 106 / ml, (221.4 ± 75.6) × 106 / ml, P <0.05] and the survival rates [(26.5 ± 2.0)%, (35.5 ± 5.9)%, (33.1 ± 5.4) %, (28.4 ± 2.1)%, P <0.01), while the high-dose and high-dose groups could significantly increase the level of serum SOD [(22.0 ± 5.3) U / ml, P <0.01] MDA content [(13.6 ± 4.6) nmol / ml, P <0.01]. The testicular histological examination showed that the proliferation of the treated group was more active than that of the blank group, showing more cells in the dividing stage. The number of mature intraluminal sperm More interstitial sparse, rich in blood vessels, interstitial cells developed well. Conclusion: Yixintangtai can improve the spermatogenic function of aged SD rats by affecting the content of SOD and MDA.