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目的探讨临床试剂盒是否适用于猪心肌梗死模型心肌肌钙蛋白(cTnI)的检测,其检测的cTnI峰值与DE-MRI所检测心肌梗死积分的关系。材料与方法 10只制备成功的猪急性心肌梗死模型分别于术前、术后1h、2h、5h、24h、48h、72h、1周采血行cTnI检测,所用试剂盒为临床试剂盒;术后行延迟增强MRI(DE-MRI)检查,心脏标本行氯化三苯基四氮唑(TTC)染色和免疫组织化学检查。结果 10例TTC染色和免疫组织化学均证实有心肌梗死;10例cTnI术后1h可见上升,于24h达高峰,24h峰值与DE-MRI、TTC检测的心肌梗死积分高度相关,相关系数分别为0.84、0.855(P值<0.05),有显著统计学意义。结论临床试剂盒对猪血清cTnI术前及术后检测,可反映猪心肌损伤;其24h峰值与DE-MRI心肌梗死范围强相关;两者均可活体内检测,且对坏死心肌有高度敏感性,两者结合有助于结果的相互印证和互为补充,可更准确地评价心肌梗死。
Objective To investigate whether clinical kit is suitable for the detection of cardiac troponin (cTnI) in porcine myocardial infarction model. The relationship between cTnI peak and the myocardial infarction score detected by DE-MRI is discussed. MATERIALS AND METHODS Ten cetinoma models of acute myocardial infarction (AMI) were successfully established for cTnI detection at preoperative, postoperative 1h, 2h, 5h, 24h, 48h, 72h and 1 week respectively. The kit used was a clinical kit. Delayed enhanced MRI (DE-MRI) examination, heart specimens were triphenylchrotoluene (TTC) staining and immunohistochemical examination. Results Ten cases of myocardial infarction were confirmed by TTC staining and immunohistochemistry. Ten cases of cTnI increased at 1 hour and reached the peak at 24 hours. The peak of 24h was highly correlated with myocardial infarction scores detected by DE-MRI and TTC. The correlation coefficients were 0.84 , 0.855 (P <0.05), with significant statistical significance. Conclusion The results of preoperative and postoperative examination of porcine serum cTnI by clinical kit can reflect the myocardial injury in pigs. The peak value of 24h is strongly correlated with the range of DE-MRI myocardial infarction. Both of them can be detected in vivo and highly sensitive to necrotic myocardium , The combination of the two can help to confirm the results and complement each other, can be more accurately evaluate the myocardial infarction.