论文部分内容阅读
目的探讨人心房利尿钠肽(human atrial natriuretic peptide,hANP)对油酸诱导大鼠肺部炎症反应的影响。方法 18只SD大鼠,随机分为3组各6只,麻醉完成,建立呼吸机、心电监测,稳定30min后对照组单纯给予生理盐水1mL/kg,肺损伤组给予油酸0.1mL/kg,油酸-hANP组给予油酸0.1mL/kg,并于油酸注射30min后静脉注射hANP0.1g/(kg·min);油酸注射6h后,3组采用定量ELISA检测血浆及肺组织中白细胞介素-1β,-6,-10及肿瘤坏死因子-α水平。结果肺损伤组血浆与肺组织白细胞介素-1β,-6,-10与肿瘤坏死因子-α水平明显高于对照组与油酸-hANP组(P<0.05);油酸-hANP组高于对照组(P<0.05)。结论 hANP可减轻油酸诱导大鼠肺部炎症反应。
Objective To investigate the effect of human atrial natriuretic peptide (hANP) on oleosane-induced pulmonary inflammatory response in rats. Methods Eighteen Sprague-Dawley rats were randomly divided into three groups with six rats. After anesthesia was completed, ventilator and electrocardiogram (ECG) were established. After 30 minutes of stabilization, rats in the control group were given normal saline (1 mL / kg) , And the oleic acid-hANP group was given 0.1 mL / kg oleic acid, and hANP 0.1 g / (kg · min) was injected intravenously 30 min after oleic acid injection. After 6 h oleic acid injection, the three groups were detected by quantitative ELISA in plasma and lung tissue Interleukin-1β, -6, -10 and tumor necrosis factor-α levels. Results The level of interleukin-1β, -6, -10 and tumor necrosis factor-α in plasma and lung tissue in lung injury group were significantly higher than those in control group and oleic acid-hANP group (P <0.05) Control group (P <0.05). Conclusion hANP can reduce oleic acid-induced pulmonary inflammatory response in rats.