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目的评估自体细胞因子诱导杀伤细胞(CIK)回输治疗中晚期消化道恶性肿瘤效果。方法选择2012年6月～2013年1月合肥凤凰肿瘤医院生物免疫治疗中心治疗的中晚期消化道恶性肿瘤患者118例,采用自体单个核细胞制备CIK细胞,培养2～3周后给予静脉输注治疗。通过荧光活化细胞分选法(FACS)测定患者治疗前后的免疫状态、生存质量(Karnofsky评分)、疾病控制率(DCR),观察毒副作用。结果经培养后,CIK细胞扩增较好,达到预期质量标准。患者治疗后CD4+、CD8+细胞比率较治疗前增加,差异有统计学意义(P<0.05);治疗后CD3+、CD4/CD8+,CIK细胞(CD3+/CD56+)高于治疗前,差异有高度统计学意义(P<0.01)。Kamofsky评分升高>20分者27例,10～<20分者29例,<10分者36例;下降<10分者26例。食管癌、胃癌、结肠癌DCR分别为55.26%(21/38)、64.91%(327/57)、65.22%(15/23)。无心、肝、肺、肾脏中毒反应。结论 CIK治疗恶性消化道肿瘤安全可靠,副作用小,疗效确切,并能有效改善患者细胞免疫状态,提高患者的生存质量,抑制肿瘤增长,提高治疗效果。
Objective To evaluate the efficacy of autologous cytokine-induced killer (CIK) infusion in the treatment of advanced gastrointestinal malignancies. Methods 118 patients with advanced gastrointestinal malignant tumor treated by biological immunotherapy center of Hefei Phoenix Cancer Hospital from June 2012 to January 2013 were treated with autologous mononuclear cells to prepare CIK cells. After 2 to 3 weeks of culture, intravenous infusion treatment. The immune status, quality of life (Karnofsky score) and disease control rate (DCR) before and after treatment were measured by fluorescence activated cell sorting (FACS) to observe the side effects. Results After culture, CIK cells expanded better, reaching the expected quality standards. The percentage of CD4 + and CD8 + cells in patients after treatment was significantly higher than that before treatment (P <0.05). The levels of CD3 +, CD4 / CD8 + and CIK cells (CD3 + / CD56 +) after treatment were significantly higher than those before treatment (P <0.01). There were 27 cases with Kamofsky score increased> 20 points, 29 cases with 10 ~ <20 points, 36 cases <10 points and 26 cases with <10 points decreased. The DCR of esophageal cancer, gastric cancer and colon cancer were 55.26% (21/38), 64.91% (327/57) and 65.22% (15/23) respectively. Heartless, liver, lung, kidney poisoning reaction. Conclusion CIK is safe and reliable in treating malignant digestive tract tumors with small side effects and curative effect. It can effectively improve cellular immune status, improve patients’ quality of life, inhibit tumor growth and improve therapeutic effect.