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作者根据被吞噬的铁粉粒子残留磁性衰减曲线评价肺泡巨噬细胞的吞噬功能,方法如下: 用支气管肺泡灌洗回收的5×10~6个肺泡巨噬细胞(AM),培养约两小时,在塑料皿底部贴壁。然后将铁粉粒子(Fe_3O_4)0.5 mg加入皿内,按30分、1小时、两小时进行800高斯10秒磁化。磁化后立即将塑料皿移于磁通计上,测定3分钟铁粉的
The authors evaluated the phagocytic function of alveolar macrophages based on the residual magnetic decay curve of the iron particles that were swallowed, as follows: 5 × 10 ~ 6 alveolar macrophages (AM) recovered by bronchoalveolar lavage were cultured for about two hours, Affixed to the bottom of the plastic dish. Then iron powder particles (Fe_3O_4) 0.5 mg added to the dish, 30 minutes, 1 hour, two hours to 800 gauss 10 seconds magnetization. Immediately after magnetization, the plastic dish was moved on the fluxmeter to measure the iron powder for 3 minutes