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目的:通过高温环境下有氧运动引起高脂饲养大鼠血液生化指标含量变化及其对内脏白色脂肪组织TNF-α、Leptin、PPARγ、visfatin基因表达的影响,探讨脂肪细胞分化及高温有氧运动减肥的分子机制。方法:高脂饲养大鼠建模8周后,随机分成非运动对照组、23℃常温有氧运动组、35℃高温有氧运动组,每天45min连续8周中等强度跑台运动。采用全自动生化分析仪检测生化指标,RT-PCR方法检测脂肪细胞分化相关调控基因的表达。结果:运动组比非运动对照组大鼠TC、TG、LDL-C含量明显降低,而HDL-C含量升高;HS组大鼠TG、LDL-C含量较NS组低,而HDL-C含量较高;运动组组间TC含量无统计学意义。运动组比对照组大鼠脂肪组织TNF-αmRNA、visfat-inmRNA的表达明显降低,而LeptinmRNA、PPARγmRNA的表达升高;HS组LeptinmRNA、PPARγmRNA的表达较NS组明显升高,而TNF-αmRNA、visfatinmRNA的表达下降。结论:有氧运动可以有效改善高脂饲养大鼠造成的脂质代谢紊乱,且高温有氧运动促进脂质代谢的生理效应优于常温有氧运动;有氧运动对脂肪细胞抑制分化与促进分化基因表达的效应均不一致,其可能机制:有氧运动对脂肪细胞分化进行局部调控,或某些基因在脂肪细胞分化调控中占主导作用;高温有氧运动对脂肪细胞分化的调节趋势比常温条件下更为明显,其机制有待进一步研究。
OBJECTIVE: To investigate the effects of aerobic exercise on blood biochemical parameters and the expression of TNF-α, Leptin, PPARγ and visfatin in visceral white adipose tissue induced by aerobic exercise in high-temperature environment and to explore the role of adipocyte differentiation and hyperthermia aerobic exercise The molecular mechanism of weight loss. Methods: After 8 weeks of high fat diet, the rats were randomly divided into non-exercise control group, 23 ℃ aerobic exercise group and 35 ℃ high temperature aerobic exercise group. Biochemical indicators were detected by automatic biochemical analyzer, and the expression of regulatory genes related to adipocyte differentiation was detected by RT-PCR. Results: Compared with non-exercise control group, TC, TG and LDL-C in exercise group decreased significantly, while HDL-C content increased in HS group. TG and LDL-C in HS group were lower than those in NS group Higher; exercise group between the TC content was not statistically significant. Compared with the control group, the expression of TNF-αmRNA and visfat-inmRNA in adipose tissue of rats in exercise group decreased significantly, while the expression of LeptinmRNA and PPARγmRNA increased in HS group. The expression of Leptin mRNA and PPARγmRNA in HS group was significantly higher than that in NS group The decline of expression. Conclusion: Aerobic exercise can effectively improve the lipid metabolism disorders caused by high fat diet rats, and the physiological effects of hyperbaric aerobic exercise on lipid metabolism are better than aerobic exercise at normal temperature. Aerobic exercise can inhibit the differentiation and promote the differentiation of adipocytes The possible mechanisms are: aerobic exercise plays a role in local regulation of adipocyte differentiation or certain genes play a dominant role in the regulation of adipocyte differentiation; the regulation tendency of adipocyte differentiation under high temperature aerobic exercise is higher than normal temperature condition Under the more obvious, the mechanism needs further study.