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利用 1 1对SSR引物对 2 4个花生栽培品种 (包括四大类型 )进行PCR扩增分析 ,其中 4对检测到明显的多态性 ,共检测到 3 3个等位基因变异 ,每一个位点上检测到的等位变异数为 5～ 1 3个 ,平均为 8.2 5个。根据扩增结果可以将 2 4个品种中的 2 1个相互区分。供试品种间的遗传相似系数值为 0 .2～ 1 .0 ,平均为 0 .4 788。根据UPGMA聚类分析结果 ,供试品种大多数按亚种聚为两大类群 (Ⅰ、Ⅱ ) ;在两大类群下 ,大多数品种也基本上按类型分类。本研究结果表明 ,SSR在分析栽培种花生DNA多态性和遗传关系方面非常有用
A total of 24 peanut cultivars (including four types) were amplified by PCR using 11 pairs of SSR primers. Of the 4 pairs, significant polymorphisms were detected, of which 33 alleles were detected. Each allele The number of alleles detected on the spot was 5 to 13 with an average of 8.25. Based on the amplification results, 21 of the 24 varieties can be distinguished from each other. The genetic similarity coefficient of tested cultivars ranged from 0.2 to 1.0, with an average of 0.478. According to the results of UPGMA cluster analysis, most of the tested cultivars were sub-clustered into two major groups (Ⅰ, Ⅱ). Under the two major groups, most cultivars were also classified by type. The results of this study indicate that SSR is very useful in analyzing the genetic diversity and genetic relationship of cultivated peanut DNA