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肾小球膜细胞过度增殖是肾小球肾炎(包括IgA肾小球肾炎)主要组织学变化之一。以往研究发现,口服紫苏煎液对40周龄以上ddY小鼠自发性IgA肾小球肾炎的炎症发展具有抑制作用。根据紫苏煎液对体外培养的小鼠肾小球膜细胞增殖的抑制作用,从紫苏70％丙酮提取物中分离出4种有效成分。本次研究紫苏煎液(热水提取物)中抑制肾小球肾炎的有效成分,并测定不同化学型的紫苏中有效成分的含量。 将紫苏煎液400mL减压浓缩至50mL,上HP-20柱层析,并用水、50％及100％甲
The excessive proliferation of mesangial cells is one of the major histological changes in glomerulonephritis (including IgA glomerulonephritis). Previous studies have found that oral basil decoction has inhibitory effects on the inflammatory development of spontaneous IgA glomerulonephritis in ddY mice over 40 weeks of age. According to the inhibitory effect of perilla decoction on the proliferation of mouse mesangial cells cultured in vitro, 4 active ingredients were isolated from 70% acetone extract of perilla. In this study, the effective components of glomerulonephritis were inhibited in perilla frying liquid (hot water extract), and the content of active ingredients in different chemical types of perilla was determined. 400 mL of Perilla decoction was concentrated under reduced pressure to 50 mL, and purified by HP-20 column chromatography with water, 50% and 100% A.