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目的 探讨新的方法检测ＡＦＰ异质体（ＡＦＰ－Ｌ３）和γ－ＧＴⅡ对原发性肝癌（ＰＨＣ）的诊断价值。方法 用抗体亲和印迹法检测ＡＦＰ－Ｌ３，并结合γ－ＧＴⅡ定性、定量检测了１１５例各种肝病及消化道肿瘤患者血清。结果 γ-ＧＴⅡ定量检测的敏感性和特异性最高，其次为γ－ＧＴⅡ定性及ＡＦＰ－Ｌ３，联合检测可以提高ＰＨＣ，尤其是小肝癌及低浓度ＡＦＰ肝癌的检出率。结论 ＡＦＰ－Ｌ３、γ－ＧＴⅡ是诊断ＰＨＣ敏感性和特异性较高的指标，对小肝癌及低浓度ＡＦＰ肝癌具有重要意义，联合检测可以提高ＰＨＣ的检出率，可以弥补ＡＦＰ的不足，有助于ＰＨＣ的早期诊断。
Objective To explore a new method for the detection of AFP-L3 and γ-GTII in the diagnosis of primary liver cancer (PHC). Methods AFP-L3 was detected by antibody affinity blotting and qualitative and quantitative detection of serum from 115 patients with various liver and digestive tract tumors was performed in combination with γ-GTII. Results The γ-GTII quantitative assay had the highest sensitivity and specificity, followed by γ-GTII characterization and AFP-L3. Combined detection could increase the detection rate of PHC, especially small hepatocellular carcinoma and low concentration AFP hepatic carcinoma. Conclusions AFP-L3 and γ-GTII are important indicators for diagnosing the sensitivity and specificity of PHC, and have important significance for small hepatocellular carcinoma and low-concentration AFP hepatic carcinoma. Combined detection can increase the detection rate of PHC and can compensate for the deficiency of AFP. Helps early diagnosis of PHC.