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目的:研究巴曲酶对沙土鼠脑缺血再灌注(Ir)期间海马存活锥体细胞数目,神经行为学,海马ATP和羟自由基含量的影响。方法:阻断沙土鼠双侧颈总动脉造成前脑缺血10min。高效液相测定海马ATP和2,3-DHBA的含量。结果:Ir组沙土鼠脑缺血后d7存活锥体细胞数(7±4)％少于Bat组的(45±16)％。在脑缺血后d3和d6时,Ir组沙土鼠的探究活动次数多于Bat组的120％和140％。在再灌注60min时,Ir组沙土鼠ATP水平低于Bat Ⅰ和Ⅱ组(82％和89％),但2,3-DHBA的含量高于Bat Ⅰ和Ⅱ组(2.6和2.4倍)。结论:巴曲酶具有减少脑缺血再灌注后延迟性神经元死亡和羟自由基产生的作用。
Objective: To study the effects of batroxobin on the number of hippocampal viable pyramidal neurons, neurobehavioral changes and ATP and hydroxyl free radicals in hippocampus during cerebral ischemia-reperfusion (Ir) in gerbils. Methods: The occlusion of bilateral carotid arteries in gerbils caused forebrain ischemia for 10 minutes. Hippocampal ATP and 2,3-DHBA levels were determined by HPLC. Results: The number of surviving pyramidal cells on d7 was less than that of the Bat group (45 ± 16)% in the Ir group. At d3 and d6 after cerebral ischemia, the number of explorations of gerbils in Ir group was more than that in Bat group by 120% and 140%, respectively. At 60 min after reperfusion, the level of ATP in gerbils of Ir group was lower than that of Bat Ⅰ and Ⅱ (82% vs 89%), while the content of 2,3-DHBA was higher than that of Bat Ⅰ and Ⅱ (2.6 and 2.4 times). Conclusion: Batroxobin has the effect of reducing delayed neuronal death and hydroxyl radical production after cerebral ischemia-reperfusion.