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目的:研究虎杖提取物对大鼠局灶性脑缺血再灌注炎性损伤的保护作用。方法:将SD大鼠随机分为6组ig给药,假手术组和模型组给予等量生理盐水;尼莫地平组按1 mg.kg-1给予尼莫地平;虎杖提取物低、中、高剂量组分别按5,10,20 mg.kg-1给予虎杖提取物。给药7 d后制作大鼠大脑中动脉闭塞模型,观察大鼠行为学改变;取大脑并用氯化四唑染色后测定梗死百分比和含水量;进行大脑组织学检查,制作脑匀浆测定白介素(IL)-1β,IL-6,IL-8和一氧化氮(NO)的含量。结果:虎杖提取物高、中剂量组的行为学评分分别为(2.6±0.9),(2.2±1.1)分;梗死率分别为(37.6±1.9)%,(30.2±4.2)%;脑含水量分别为(78.1±5.6)%,(71.2±3.9)%,均较模型组显著降低(P<0.01);IL-1β分别为(0.64±0.28),(0.65±0.31)μg.L-1;IL-6分别为(7.2±2.7)ng.L-1,(8.8±2.2)ng.L-1,IL-8分别为(12.6±2.5)μg.L-1,(15.2±2.1)μg.L-1和NO分别为(2.9±1.3),(3.2±1.1)mmol.L-1,较模型组均明显降低(P<0.05,P<0.01)。结论:虎杖提取物对脑缺血再灌注炎性损伤有一定的保护作用。
Objective: To study the protective effect of polygonum cuspidatum extract against inflammatory injury induced by focal cerebral ischemia-reperfusion in rats. Methods: The SD rats were randomly divided into 6 groups. The rats in the sham operation group and model group were given the same amount of normal saline. The nimodipine group was given nimodipine at 1 mg.kg-1. High dose group were given 5,10,20 mg.kg-1 Polygonum cuspidatum extract. After 7 d administration, middle cerebral artery occlusion model was made in rats, and behavioral changes were observed. The brain was harvested and infarct percentage and water content were determined by tetrazolium chloride staining. Cerebral histological examination was performed to determine brain interleukin IL-1β, IL-6, IL-8 and nitric oxide (NO). Results: The behavioral scores of Polygonum Cuspidatum extract group were (2.6 ± 0.9) and (2.2 ± 1.1), respectively; the infarct rates were (37.6 ± 1.9) and (30.2 ± 4.2)% (78.1 ± 5.6)% and (71.2 ± 3.9)% respectively, which were significantly lower than those in the model group (P <0.01). The levels of IL-1β were (0.64 ± 0.28) and (0.65 ± 0.31) The levels of IL-6 were (7.2 ± 2.7) ng.L-1 and (8.8 ± 2.2) ng.L-1 and (12.6 ± 2.5) μg.L-1 and (15.2 ± 2.1) μg respectively. L-1 and NO were (2.9 ± 1.3) and (3.2 ± 1.1) mmol.L-1, respectively, which were significantly lower than those in model group (P <0.05, P <0.01). Conclusion: Polygonum cuspidatum extract can protect cerebral ischemia-reperfusion injury.