论文部分内容阅读
目的:利用聚合物纳米微球材料分离纯化金丝桃素。方法:采用PEK微球对金丝桃素提取物进行富集,运用PDCX微球纯化,通过ODS柱制备高纯度金丝桃素,通过单因素试验优选纯化工艺。采用HPLC测定金丝桃素含量,流动相甲醇-0.006 mol·L-1Na2HPO4(7∶1,加H3PO4调pH 6.5),检测波长590 nm。结果:PEK微球对金丝桃素的静态饱和吸附量8.5 mg·g-1,解析率89.6%,经PEK微球动态柱富集的金丝桃素样品纯度3.2%,回收率89.9%;经PDCX微球纯化后,金丝桃素纯度33.0%,回收率80.1%;经ODS柱制备的金丝桃素纯度达91.6%,回收率86.7%。结论:该制备工艺高效、环保、经济,适用于金丝桃素等不稳定性成分的分离纯化。
Objective: To isolate and purify hypericin from polymer nanospheres. Methods: The hypericin extract was enriched by PEK microspheres and purified by PDCX microspheres. High purity hypericin was prepared by ODS column. The purification process was optimized by single factor test. The content of hypericin was determined by HPLC. The mobile phase consisted of methanol-0.005 mol·L-1Na2HPO4 (7:1, H3PO4 adjusted to pH 6.5). The detection wavelength was 590 nm. Results: The saturated adsorption capacity of hypericin to PEK microspheres was 8.5 mg · g-1, the resolution was 89.6%. The purity of hypericin sample collected by PEK microspheres dynamic column was 3.2% and the recovery was 89.9%. Purified by PDCX microspheres, the purity of hypericin was 33.0% and the recovery was 80.1%. The purity of hypericin prepared by ODS column was 91.6% with the recovery of 86.7%. Conclusion: The preparation process is efficient, environmentally friendly and economical. It is suitable for the separation and purification of unstable components such as hypericin.