论文部分内容阅读
‘Hort 16A'猕猴桃是继‘Hayward'之后的第二代猕猴桃优良品种,备受消费者青睐.其后代遗传了母本大多数的优良品质,但其遗传分化及后代优株分子标记的研究还未见相关报道.本研究将24 株具有优良性状的‘Hort 16A'猕猴桃自由授粉后代,通过优化酶切、连接、预扩增、选择性扩增等步骤建立AFLP 反应体系,并利用Popgene 和NTSYS 分别对遗传多样性参数和个体聚类进行分析.结果表明,筛选出的5 对多态性较高的选择性扩增引物,共扩增出426 个AFLP 标记,多态性标记424 个,多态性标记百分比达到 99.53％.不同优良株系在M-CAC/E-AGC 引物对上均有特异性位点,这些特异位点用于申报优良品种保护.总基因多样度(Ht)为0.300 0,居群内基因多样度(Hs)为0.289 8,遗传分化系数(Gst)为0.030 2,基因流(Nm)为 14.101 8.遗传分化系数远小于0.15,而基因流远大于4.在通过NTSYS 软件聚类后,在遗传相似系数为0.51时,将24 个个体分为2 个大支.本研究的结果多态性比例很高,对样品的区分率达到100％,为猕猴桃AFLP分析检测提供了很大应用和指导借鉴价值.“,”‘Hort 16A' kiwi fruit is the good variety of the second generation after 'Hayward',which is favored by consumers.Its offspring inherits most of the excellent qualities of maternal parents,but researches on their genetic differentiation and the molecular markers of the offspring haven't been reported yet.The study established the AFLP reaction system based on the optimization of enzyme,connection,preamplification,selective amplification and other steps of 24 open-pollinated progenies of ‘Hort 16A' kiwi fruit with excellent qualities,and Popgene and NTSYS were employed to analyze the genetic diversity parameters and individual clustering,respectively.The results showed that five pairs of highly polymorphic selective amplification primers screened amplified a total of 426 AFLP markers,including 424 polymorphic markers,in which the percentage of polymorphic markers reached 99.53％.Different strains all had specific sites in M-CAC/E-AGC primers,and these specific sites were used to declare good varieties protection.The total degree of genetic diversity (Ht) was 0.300 0.The intra-population gene diversity Hs was 0.289 8.Genetic differentiation coefficient (Gst) was 0.030 2,and gene flow Nm was 14.101 8.The genetic differentiation coefficient was much less than 0.15,and gene flow was much larger than 4.After clustering by NTSYS software,24 individuals were divided into two large branches when the genetic similarity coefficient was at 0.51.The results of this study was high in polymorphism,and the discrimination rate of samples was up to 100％,which might provide great application and reference value for the analysis and detection of the AFLP in kiwi fruit.