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目的:探讨微小核糖核苷酸(miRNA)在急性心肌梗死(AMI)早期诊断中的价值。方法:将发病时间<3h的胸痛患者60例根据其诊断结果分为AMI组(n=30)和非AMI组(n=30),另选择正常对照组(n=30),在入院即刻测定mi R-208a、肌钙蛋白、肌红蛋白、磷酸肌酸心肌同工酶(CK-MB),比较各组生化标记物的变化。在AMI组动态监测miR-208a和肌钙蛋白的波动情况;以实时荧光定量聚合酶链反应测定miR-208a。结果 :入院即刻AMI组miR-208a明显高于对照组(P<0.05),但肌钙蛋白和CK-MB与对照组无明显差异;发病1h时的miR-208a显著高于对照组(P<0.05),肌钙蛋白浓度尚处于正常范围内。miR-208a的峰值出现于发病12h,峰值明显早于肌钙蛋白。结论:miR-208a在早期AMI诊断中具备高特异性和高敏感性。
Objective: To investigate the value of miRNA in the early diagnosis of acute myocardial infarction (AMI). Methods: Sixty cases of chest pain with morbidity less than 3 hours were divided into AMI group (n = 30) and non-AMI group (n = 30) according to the diagnosis results. The control group (n = 30) mi R-208a, troponin, myoglobin and creatine phosphokinase (CK-MB) were measured. The changes of biochemical markers in each group were compared. In the AMI group, the fluctuation of miR-208a and troponin were dynamically monitored; miR-208a was determined by real-time fluorescence quantitative polymerase chain reaction. Results: miR-208a in AMI group was significantly higher than that in control group immediately after admission (P <0.05), but there was no difference in troponin and CK-MB between control group and AMI group. 0.05), troponin concentration is still within the normal range. The peak of miR-208a appeared in the onset of 12h, the peak was significantly earlier than troponin. Conclusion: miR-208a is highly specific and sensitive in the early diagnosis of AMI.