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甜菜坏死黄脉病毒(BNYVV)及其新变异株P型和IV-A型仍对甜菜生产造成重大损失。选育抗病品种是防治该病的唯一有效措施。用ELISA和实时定量逆转录PCR技术测定BNYVV和P.betae可对杂交后代分离群体或转基因后代中的抗病个体进行选择。AFLP、RAPD和PCR等分子标记的建立可对抗病基因位点Rz1(Holly)、Rz2(WB42)、Rz3(WB41)和Rz4(C50,R22)进行辅助选择。在分离植物抗病基因方面,已获得含Rz1的BAC克隆和候选基因。转基因甜菜含重复倒置的BNYVV复制酶基因、RNA-2转运蛋白P15突变基因或CP基因显著提高了抗病性。国际合作可有效地利用资源,加快抗病种质创新。
Beet necrotic yellow vein virus (BNYVV) and its new variants P and IV-A still cause significant losses in sugar beet production. Breeding of resistant varieties is the only effective way to control the disease. Determination of BNYVV and P.betae by ELISA and Real-Time Quantitative Reverse Transcription PCR Techniques Selection of resistant individuals in isolated offspring or transgenic progeny of hybrids can be performed. The establishment of molecular markers such as AFLP, RAPD and PCR assisted the selection of disease loci Rz1 (Holly), Rz2 (WB42), Rz3 (WB41) and Rz4 (C50, R22). In the isolation of plant disease resistance genes, BAC clones containing Rz1 and candidate genes have been obtained. Transgenic beets contain repeatedly inverted BNYVV replicase gene, RNA-2 transporter P15 mutant gene or CP gene significantly improves disease resistance. International cooperation can make effective use of resources to speed up disease-resistant germplasm innovation.