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目的：在染色体 3p14.2- 14.3区域寻找与鼻咽癌发生发展密切相关的新基因。方法：运用 RACE方法获取基因全长 cDNA序列， pEGFP质粒与脂质体共转染 COS7细胞进行新基因的蛋白质定位。结果：在 3p14.2- 14.3区域克隆了一个在鼻咽癌中表达下调新基因的全长 cDNA序列，被命名为 NPCDR1，编码 109个氨基酸，其蛋白质聚集在细胞核内，其基因组由 2个外显子和 1个内含子组成。 Northern印迹杂交发现鼻咽癌组织中存在异常转录本。突变检测发现单碱基多态性改变。结论： NPCDR1基因可能是一个新的候选抑瘤基因，其表达下调可能与鼻咽癌的发生发展有关。
OBJECTIVE: To search for new genes closely related to the occurrence and development of nasopharyngeal carcinoma on chromosome 3p14.2-14.3. Methods: The full-length cDNA sequence was obtained by RACE method. The pEGFP plasmid and liposome were co-transfected into COS7 cells for protein localization. RESULTS: A full-length cDNA sequence encoding a new gene down-regulated in NPC was cloned in the region of 3p14.2-14.3 and named NPCDR1 encoding a protein of 109 amino acids. Its protein is aggregated in the nucleus and its genome consists of two Exons and an intron composition. Northern blotting revealed abnormal transcripts in nasopharyngeal carcinoma. Mutations detected single base polymorphism. Conclusion: NPCDR1 gene may be a new candidate tumor suppressor gene. The down-regulation of NPCDR1 gene may be related to the occurrence and development of nasopharyngeal carcinoma.