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目的:通过对2010年苏州地区手足口病病例进行病原体检测并进行流行特征分析,为手足口病的防控提供科学依据,同时评估实时荧光定量PCR在手足口病防控中的应用价值。方法:临床诊断为手足口病病例的咽拭子标本1711份,首先采用实时荧光定量PCR筛选出总肠道病毒阳性的标本,再分别使用CA16和EV71特异性引物进行荧光定量PCR检测。结果:手足口病全年均可发病,以4月-7月为发病高峰季节。发病率最高的年龄段为1岁～4岁,男性多于女性。重症病例主要由EV71引起。结论:苏州市的手足口病毒以EV71为主。实时荧光定量PCR法可用于手足口病的病原体的快速检测和分型,有利于感染者的早发现、早隔离,对手足口病的监测及防控具有重要作用。
Objective: To provide a scientific basis for the prevention and control of HFMD by pathogen detection and epidemiological analysis of HFMD in Suzhou area in 2010, and to evaluate the value of real-time PCR in the prevention and control of HFMD. Methods: A total of 1711 throat swabs were diagnosed as hand-foot-mouth disease. First, real-time fluorescence quantitative PCR was used to screen out the positive samples of total enteroviruses. The specific primers of CA16 and EV71 were respectively used for quantitative PCR detection. Results: Hand, foot and mouth disease can be disease throughout the year, April to July peak season for the incidence. The highest incidence of 1 to 4 years old age group, more men than women. Severe cases are mainly caused by EV71. Conclusion: EV71 is the main hand-foot-and-mouth disease virus in Suzhou. Real-time PCR method can be used for the rapid detection and typing of pathogens of hand-foot-mouth disease, which is beneficial to the early detection and early isolation of the infected person and the monitoring and prevention and control of foot-mouth disease.