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目的调查兰州地区166例喘息性疾病患儿的病毒病原谱构成。方法收集毛细支气管炎、喘息性支气管肺炎及婴幼儿哮喘患儿的鼻咽抽吸物166份,应用PCR及RT-PCR方法检测常见4种DNA病毒(人腺病毒、人博卡病毒、人多瘤病毒WU、人多瘤病毒KI)和6种RNA病毒(人呼吸道合胞病毒、人鼻病毒、人偏肺病毒、人流感病毒、人副流感病毒、人冠状病毒HKU1)。结果166份鼻咽抽吸物标本中,病毒检测阳性143份,检出率为86.14%。人呼吸道合胞病毒、人鼻病毒、人偏肺病毒、人流感病毒、人副流感病毒、人冠状病毒HKU1、人腺病毒、人博卡病毒、多瘤病毒KI和多瘤病毒WU检出率分别为51.81%、23.49%、5.42%、6.02%、10.84%、3.01%、15.66%、14.46%、8.43%和4.82%。结论病毒性致病原是导致喘息性疾病的主要致病原,主要为人呼吸道合胞病毒、人鼻病毒、人腺病毒等。
Objective To investigate the virus pathogenic spectrum of 166 children with asthmatic disease in Lanzhou area. Methods A total of 166 nasopharyngeal aspirates were collected from patients with bronchiolitis, asthmatic bronchial pneumonia and infantile asthma. Four common DNA viruses (human adenovirus, human Boka virus, human Neoplasia virus WU, human polyomavirus KI) and six RNA viruses (human respiratory syncytial virus, human rhinovirus, human metapneumovirus, human influenza virus, human parainfluenza virus, human coronavirus HKU1). Results Of the 166 samples of nasopharyngeal aspirates, 143 were positive for the virus and the detection rate was 86.14%. Human respiratory syncytial virus, human rhinovirus, human metapneumovirus, human influenza virus, human parainfluenza virus, human coronavirus HKU1, human adenovirus, human boka virus, polyomavirus KI and polyoma virus WU detection rate Respectively 51.81%, 23.49%, 5.42%, 6.02%, 10.84%, 3.01%, 15.66%, 14.46%, 8.43% and 4.82%. Conclusions Viral pathogens are the main causative agents of asthmatic diseases, mainly human respiratory syncytial virus, human rhinovirus and human adenovirus.