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目的:观察N-myc下游调节基因1(N-myc downstream regulated gene1,NDRG1)、P21及血管内皮生长因子(vascular endothelial growth factor,VEGF)基因在肺腺癌中的表达及在肺腺癌发生、发展中的作用及意义。方法:应用免疫组织化学法检测20例正常肺组织和94例肺腺癌组织中NDRG1、P21及VEGF蛋白的表达情况,并分析三者表达与临床病理参数及患者预后的相关性。结果:在正常肺组织及肺腺癌组织中,NDRG1阳性率分别为90.0%(18/20)和52.1%(49/94),P21阳性率分别为80.0%(16/20)和33.0%(31/94),VEGF阳性率分别为20.0%(4/20)和53.2%(50/94),3种蛋白阳性率比较差异均具有统计学意义(P均<0.05)。肺腺癌组织中NDRG1、P21及VEGF的表达与肺腺癌患者年龄、性别、组织分化程度及TNM分期均无相关性(P均>0.05),而与淋巴结转移明显相关(P<0.05)。Kaplan-Meier生存分析显示,NDRG1、P21和VEGF表达与生存明显相关(P均<0.01)。结论:肺腺癌组织中NDRG1和P21表达下调,而VEGF表达上调,联合检测三者的表达可能对判断预后具有重要价值。
OBJECTIVE: To observe the expression of N-myc downstream regulated gene 1 (NDRG1), P21 and vascular endothelial growth factor (VEGF) in lung adenocarcinoma, The role of development and its significance. Methods: The expressions of NDRG1, P21 and VEGF proteins in 20 normal lung tissues and 94 lung adenocarcinoma tissues were detected by immunohistochemistry. The correlation between the expression of these three proteins and clinical pathological parameters and prognosis was analyzed. Results: The positive rates of NDRG1 in normal and lung adenocarcinomas were 90.0% (18/20) and 52.1% (49/94), respectively. The positive rates of P21 were 80.0% (16/20) and 33.0% 31/94). The positive rates of VEGF were 20.0% (4/20) and 53.2% (50/94), respectively. There was significant difference in the positive rates of all the three proteins (all P <0.05). The expression of NDRG1, P21 and VEGF in lung adenocarcinoma was not correlated with the age, sex, histological differentiation and TNM stage (P> 0.05), but significantly correlated with lymph node metastasis (P <0.05). Kaplan-Meier survival analysis showed that the expressions of NDRG1, P21 and VEGF were significantly correlated with survival (P <0.01). Conclusion: The expression of NDRG1 and P21 in lung adenocarcinoma is down-regulated while the expression of VEGF is up-regulated. The combined detection of these three expressions may have important value in judging prognosis.