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目的对某饭店一起食物中毒病原菌进行微生物鉴定与药敏分析。方法参考GB/T4789-2003、GB4789-2010进行操作,采用PCR方法快速检测增菌后的目标菌,利用全自动微生物鉴定及药敏分析仪进行生化及药敏鉴定。结果19份标本有4份病人肛拭子和1份蛋糕检出布利丹沙门菌,其他目标菌均未检出,对氨苄西林、阿莫西林、氧哌嗪青霉素、头孢三嗪、头孢吡肟、噻肟单酰胺菌素、厄他培南、亚胺培南、环丙沙星、左氧氟沙星、替加环素和甲氧苄氨嘧啶敏感。结论这次食物中毒是由布利丹沙门菌引起;应用荧光定量PCR技术快速检测病原菌,结合传统细菌培养进行分析,为流行病学调查及临床药物治疗提供实验室依据。
Objective To conduct microbial identification and drug susceptibility analysis of food poisoning pathogens in a hotel. Methods Reference GB / T4789-2003, GB4789-2010 to operate, the rapid detection of bacteria by PCR amplification of bacteria, the use of automatic microbial identification and susceptibility analyzer biochemical and susceptibility identification. Results Four specimens of 19 patients were tested for Salmonella typhi with anus swabs and 1 cake, and none of the other target bacteria were detected. Ampicillin, amoxicillin, piperacillin, ceftriaxone, ceftazidime Oxime, cefotaxime, ertapenem, imipenem, ciprofloxacin, levofloxacin, tigecycline and trimethoprim. Conclusion The food poisoning is caused by Salmonella Brendaniensis. Fluorescent quantitative PCR was used to detect pathogenic bacteria rapidly, combined with traditional bacterial culture analysis, providing laboratory evidence for epidemiological investigation and clinical drug treatment.