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目的:观察调肠消炎片对溃疡性结肠炎(UC)大鼠结肠的炎症反应及血清toll样受体4(TLR-4)浓度的影响。方法:用复合法(2,4,6-三硝基苯磺酸加乙醇)造模制备UC大鼠模型,将60只SD大鼠随机分为正常组、模型组、调肠消炎片组及柳氮磺胺吡啶(SASP)组,除正常组外,其余各组从造模后第3天开始分别每天灌胃给药1次,灌胃14天后取材观察大鼠结肠炎症反应情况及血清TLR-4浓度。结果:模型组CMDI及血清TLR-4浓度均高于正常组(P<0.01)。调肠消炎片组的CMDI及血清TLR-4浓度均低于模型组(P<0.05,P<0.01)。结论:调肠消炎片明显降低UC大鼠血清TLR-4浓度,提示其可能通过调控TLR-4信号通路,有效治疗溃疡性结肠炎模型大鼠。
Objective: To observe the effect of Tiaoxin Xiaoyan Tablet on the inflammatory reaction and serum toll like receptor 4 (TLR-4) in the colon of ulcerative colitis (UC) rats. Methods: The UC rat model was prepared by compound method (2,4,6-trinitrobenzene sulfonic acid plus ethanol), and 60 SD rats were randomly divided into normal group, model group, Sulfasalazine (SASP) group, in addition to the normal group, the other groups from the first 3 days after the start of the model were intragastrically administered once a day, gavage 14 days after the observation of rat colorectal inflammation and serum TLR- 4 concentration. Results: The concentrations of CMDI and TLR-4 in model group were higher than those in normal group (P <0.01). The concentrations of CMDI and TLR-4 in Tiaoxin Xiaoyan Tablet group were lower than those in model group (P <0.05, P <0.01). Conclusion: Tiaoxin Xiaoyan Tablet can significantly reduce the concentration of serum TLR-4 in UC rats, suggesting that TLR-4 signaling pathway may be effective in treating ulcerative colitis rats.