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利用DGGE技术,从微生物多样性的角度,结合土壤酶活性研究不同农林利用方式对围垦海涂养分特征的影响。结果表明:未利用海涂盐分含量最高,而有机质和碱解氮含量最低;相比于蔬菜地和林地,棉花地和水稻田的有机质、碱解氮、过氧化氢酶和蛋白酶活性更高。四种利用方式土壤的脲酶和磷酸酶活性差异不显著。细菌是土壤中的主要种群,其数量与土壤有机质、碱解氮等养分特征呈显著正相关,表现为水稻田、棉花地>蔬菜地、林地>未利用。DGGE图谱分析结果显示未利用土壤shannon多样性指数最高为2.81,与水稻田、棉花地较为接近,其次为蔬菜地和林地,分别为2.52和2.38。围垦利用引起土壤中的Gaetbulibacter marinus与Gillisia mitskevichiae种属变化。围垦利用后土壤质量优于未利用海涂。相比而言,种植水稻对提高土壤肥力水平,维持土壤质量较为有利。
Using DGGE technology, from the perspective of microbial diversity, combined with the activity of soil enzymes to study the impact of different agroforestry use patterns on nutrient characteristics of reclaimed seawater. The results showed that the content of organic matter and alkali-hydrolyzable nitrogen in the unutilized seafloor was the highest while the content of organic matter and alkali-hydrolyzable nitrogen was the lowest. Compared with vegetable land and forest land, the organic matter, alkaline-hydrolysis nitrogen, catalase and protease activities in cotton field and paddy field were higher. There was no significant difference in urease and phosphatase activities among the four ways of utilization. Bacterium is the main population in soil. The quantity of bacteria is significantly and positively correlated with the characteristics of nutrients such as soil organic matter, alkaline hydrolysis nitrogen and so on. The performance of the bacteria is paddy field, cotton land> vegetable land and woodland> unused. The results of DGGE showed that shannon diversity index of unused soil was the highest (2.81), which was close to paddy field and cotton land, followed by vegetable land and forest land, which were 2.52 and 2.38 respectively. Reclamation and utilization caused changes in species of Gaetbulibacter marinus and Gillisia mitskevichiae in soil. After reclamation and utilization of soil quality is better than the unused sea. In contrast, planting rice is more favorable for improving soil fertility and maintaining soil quality.